| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.211387 |
| Chromosome: | chromosome 8 |
| Location: | 1216008 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g363250 | (1 of 3) IPR000104//IPR006553 - Antifreeze protein, type I // Leucine-rich repeat, cysteine-containing subtype | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCATGTTGCTCTACCGAGTGGATGGTGCT |
| Internal bar code: | CCGCACCATCCAATACTTCGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 136 |
| LEAP-Seq percent confirming: | 99.6055 |
| LEAP-Seq n confirming: | 4040 |
| LEAP-Seq n nonconfirming: | 16 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTAAGGGTAGCTCAGCACG |
| Suggested primer 2: | GAATTTTCGCGCGGTAGTAA |