| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.211388 |
| Chromosome: | chromosome 9 |
| Location: | 5481442 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g400293 | (1 of 10) IPR029068 - Glyoxalase/Bleomycin resistance protein/Dihydroxybiphenyl dioxygenase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AACCAAGCTCCAAACGTAGTCCTGCCAATT |
| Internal bar code: | ATACGGCCGGGGTCATGAGGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 566 |
| LEAP-Seq percent confirming: | 99.6633 |
| LEAP-Seq n confirming: | 296 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACTAAAGCGCGAGAGCTGAC |
| Suggested primer 2: | AATCACCCAGCTCTTCCCTT |