Insertion junction: LMJ.RY0402.211393_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.211393
Chromosome:chromosome 14
Location:3819057
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre14.g632700 (1 of 9) 2.7.10.1 - Receptor protein-tyrosine kinase / Receptor protein tyrosine kinase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCAAGCACCACTACAACCCCCCTACATTGG
Internal bar code:GCACTAATTCTAACATTTCTAC

Confirmation - LEAP-Seq

LEAP-Seq distance:289
LEAP-Seq percent confirming:99.6305
LEAP-Seq n confirming:809
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:GGTTCTTTCCCATTTCCCAT
Suggested primer 2:CGCATTCAGCCTACAGAACA