Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.211401 |
Chromosome: | chromosome_10 |
Location: | 5162413 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre10.g456600 | antisense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | CCAGATTGGCGCTGGCCAACCCCATGCCCT |
Internal bar code: | GTGCTCCCTCGCCCCGGGCGAC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 681 |
LEAP-Seq percent confirming: | 98.7755 |
LEAP-Seq n confirming: | 242 |
LEAP-Seq n nonconfirming: | 3 |
LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CCACCTTGATGTTGTCGATG |
Suggested primer 2: | AAGGACGACAACAACTTCGG |