| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.211407 |
| Chromosome: | chromosome 3 |
| Location: | 5434294 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g185100 | (1 of 1) K15698 - RING finger protein 121 (RNF121) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AACCCTGCACTGCAAAAATCCTGTACTATA |
| Internal bar code: | ATTCTACGATGGACGACCCCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 132 |
| LEAP-Seq percent confirming: | 95.2891 |
| LEAP-Seq n confirming: | 445 |
| LEAP-Seq n nonconfirming: | 22 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCTGGCTTGTGGTATGATT |
| Suggested primer 2: | GCTTCGACTGTGGGTACCAT |