| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.211408 |
| Chromosome: | chromosome 2 |
| Location: | 4675482 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g101500 | MRS1 | Mg2+ transporter protein; (1 of 3) K16075 - magnesium transporter (MRS2, MFM1) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GACGGCGGCTACAATGGCGACGTGCACGGA |
| Internal bar code: | GGGTCGGAATGGGCCGTGCCCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 918 |
| LEAP-Seq percent confirming: | 88.9807 |
| LEAP-Seq n confirming: | 15399 |
| LEAP-Seq n nonconfirming: | 1907 |
| LEAP-Seq n unique pos: | 81 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TATTTGCGTGTTCGTCTTGC |
| Suggested primer 2: | GCTCCATGCTTTCTCAGGTC |