| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.211453 |
| Chromosome: | chromosome 7 |
| Location: | 5933721 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g354250 | PGK2 | (1 of 2) PF00162 - Phosphoglycerate kinase (PGK); Phosphoglycerate kinase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGGTCAGGTGAGAGGGCTGGGCGGACGAT |
| Internal bar code: | CACAATGGGCGCCAAGCATCTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 795 |
| LEAP-Seq percent confirming: | 99.5686 |
| LEAP-Seq n confirming: | 6001 |
| LEAP-Seq n nonconfirming: | 26 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGTTGTTTGTGGGTCTGGC |
| Suggested primer 2: | ATCACAGATGTGGACGTGGA |