| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.211466 |
| Chromosome: | chromosome 16 |
| Location: | 4068587 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g669800 | (1 of 2) K04345 - protein kinase A (PKA) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGAACCACGTGAGGCAGCAGTCGCCGCGTT |
| Internal bar code: | ACACAGGCAGACGTCGAGATTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 606 |
| LEAP-Seq percent confirming: | 99.3364 |
| LEAP-Seq n confirming: | 7634 |
| LEAP-Seq n nonconfirming: | 51 |
| LEAP-Seq n unique pos: | 47 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACTAACCGAACAGGACACCG |
| Suggested primer 2: | AACCAGTCCTGCTGCATCTT |