Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.211495 |
Chromosome: | chromosome 14 |
Location: | 273714 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre14.g609551 | 3'UTR |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGAGTCTTGACGGATCCCAGGGGTCTGCC |
Internal bar code: | CGGTTACTAGCCAAGCGACTCC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 1009 |
LEAP-Seq percent confirming: | 99.6134 |
LEAP-Seq n confirming: | 19583 |
LEAP-Seq n nonconfirming: | 76 |
LEAP-Seq n unique pos: | 90 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CCTCAATTTGGGCTGTTGTT |
Suggested primer 2: | GGTTTGCCCGCTGAGTAATA |