| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.211499 |
| Chromosome: | chromosome 2 |
| Location: | 5287498 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g106650 | CDPK7 | (1 of 6) PTHR24349//PTHR24349:SF23 - SERINE/THREONINE-PROTEIN KINASE // MAP KINASE-ACTIVATED PROTEIN KINASE 5; Calcium/calmodulin-dependent protein kinase | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGTGTCTTTAACTGACAACCAAGAAGGTC |
| Internal bar code: | GTCGTCCCGGCGGGCACGGTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 983 |
| LEAP-Seq percent confirming: | 95.328 |
| LEAP-Seq n confirming: | 6917 |
| LEAP-Seq n nonconfirming: | 339 |
| LEAP-Seq n unique pos: | 49 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCACACCATAGCTTCCCTG |
| Suggested primer 2: | CTCCGGACTCAGGATAGCTG |