| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.211501 |
| Chromosome: | chromosome 16 |
| Location: | 4101230 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g669500 | (1 of 726) IPR011009 - Protein kinase-like domain | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCCGCTGCTGCTGCTGCTGCTGCGGCGGC |
| Internal bar code: | CCGTGGGATAGATGTAGGGGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 793 |
| LEAP-Seq percent confirming: | 93.297 |
| LEAP-Seq n confirming: | 1712 |
| LEAP-Seq n nonconfirming: | 123 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCCCAGCAGCAGTAGACCTT |
| Suggested primer 2: | GCATGAATGCAAAGCGTAGA |