Insertion junction: LMJ.RY0402.211510_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.211510
Chromosome:chromosome 2
Location:2423040
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre02.g091500 (1 of 1) PTHR13595:SF1 - ADP-RIBOSYLATION FACTOR-LIKE PROTEIN 6-INTERACTING PROTEIN 4 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGAGGCCACCGCGGACGGGTCCGTGCTAAA
Internal bar code:AGTCAAGCACTTCGCATCGCTC

Confirmation - LEAP-Seq

LEAP-Seq distance:329
LEAP-Seq percent confirming:35.7599
LEAP-Seq n confirming:840
LEAP-Seq n nonconfirming:1509
LEAP-Seq n unique pos:9

Suggested primers for confirmation by PCR

Suggested primer 1:GCCATCAAGAGGTGCGTAAT
Suggested primer 2:ATGATACGATGGTGGGCAGT