| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.211511 |
| Chromosome: | chromosome_5 |
| Location: | 1202769 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre05.g245350 | antisense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | ACCAATCCTCGACCTGGCTACGCCTTCACT |
| Internal bar code: | CGTTTCAGACGTGGGGATGGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 761 |
| LEAP-Seq percent confirming: | 68.8069 |
| LEAP-Seq n confirming: | 8316 |
| LEAP-Seq n nonconfirming: | 3770 |
| LEAP-Seq n unique pos: | 71 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCGGTACAAGGGGAAGTTT |
| Suggested primer 2: | CCAGCCCAAACTAAACCAAA |