| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.211513 |
| Chromosome: | chromosome 6 |
| Location: | 1219716 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g257700 | RAD18 | Putative E3 ubiquitin ligase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGTTACGTGTCACGTATGTCTCTGTCCAC |
| Internal bar code: | GGGGCCCTAACTTCGGATTTCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 208 |
| LEAP-Seq percent confirming: | 90.9853 |
| LEAP-Seq n confirming: | 434 |
| LEAP-Seq n nonconfirming: | 43 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGCCTCTCCTCTTCCTCCT |
| Suggested primer 2: | AGGCTACTTGATGCATTGCC |