| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.211523 |
| Chromosome: | chromosome 9 |
| Location: | 5041177 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g398252 | (1 of 13) 1.1.1.300 - NADP-retinol dehydrogenase / Retinol dehydrogenase (NADP(+)) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTAGGGCGTGGGGTTACAGGCGCAGCGTG |
| Internal bar code: | TTAATCCAGAGCCCTCGAGCCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 169 |
| LEAP-Seq percent confirming: | 21.3129 |
| LEAP-Seq n confirming: | 2224 |
| LEAP-Seq n nonconfirming: | 8211 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAGGTGTCTACTGGGGGTA |
| Suggested primer 2: | GTGTTGGGTTTTGGGTTTTG |