Insertion junction: LMJ.RY0402.211531_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.211531
Chromosome:chromosome 3
Location:5622727
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre03.g187050 PHT4-3,PHT3,PHT4C (1 of 1) K12303 - MFS transporter, ACS family, solute carrier family 17 (sodium-dependent inorganic phosphate cotransporter), member 9 (SLC17A9); Na+-dependent inorganic phosphate cotransporter intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGGGAAGGGGGGGGGGTACTTTTTTCCAAT
Internal bar code:TCCTACTTTTGCACTCTCTTTG

Confirmation - LEAP-Seq

LEAP-Seq distance:593
LEAP-Seq percent confirming:87.4459
LEAP-Seq n confirming:606
LEAP-Seq n nonconfirming:87
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:ACTTGGTAGCCTCCACGATG
Suggested primer 2:GTTCCTGGTGTTCGGAGTGT