| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.211549 |
| Chromosome: | chromosome 13 |
| Location: | 1189591 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g569850 | AMT4,AMT1D | Ammonium transporter; (1 of 8) K03320 - ammonium transporter, Amt family (amt, AMT, MEP) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AACAGAGAGTCACCGATCTGTGGGGGAGGA |
| Internal bar code: | CGGGAGTTCAGCATGGGGTGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 779 |
| LEAP-Seq percent confirming: | 99.2503 |
| LEAP-Seq n confirming: | 3442 |
| LEAP-Seq n nonconfirming: | 26 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTAGACGAAGGCGGAGATGA |
| Suggested primer 2: | GACAACAGTGACCTGAGCGA |