Insertion junction: LMJ.RY0402.211561_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.211561
Chromosome:chromosome 2
Location:1063133
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre02.g080750 (1 of 274) IPR020683 - Ankyrin repeat-containing domain intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACACTCGCTCAACAGACTCGTAATTTCCTG
Internal bar code:GTCCCGATCTGTGTTTGGGGGC

Confirmation - LEAP-Seq

LEAP-Seq distance:235
LEAP-Seq percent confirming:99.5596
LEAP-Seq n confirming:2713
LEAP-Seq n nonconfirming:12
LEAP-Seq n unique pos:9

Suggested primers for confirmation by PCR

Suggested primer 1:GTGGTGTAGCCAGGAATCGT
Suggested primer 2:ACAAATGCCTTCGGCATAAC