| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.211561 |
| Chromosome: | chromosome 2 |
| Location: | 1063152 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g080750 | (1 of 274) IPR020683 - Ankyrin repeat-containing domain | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGAGCTGACACACGCGTGTCGTCCCCTCGC |
| Internal bar code: | AATGGGTGGGAGTAACATGTCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 683 |
| LEAP-Seq percent confirming: | 99.7778 |
| LEAP-Seq n confirming: | 3592 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 24 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACAAATGCCTTCGGCATAAC |
| Suggested primer 2: | GTGGTGTAGCCAGGAATCGT |