| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.211570 |
| Chromosome: | chromosome 8 |
| Location: | 4914854 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g385100 | EFH5 | EF-hand Calcium binding protein; (1 of 38) IPR002048//IPR011992 - EF-hand domain // EF-hand domain pair | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTGCCGCGCCTGCCGCGATGACCCTGTGA |
| Internal bar code: | CACGTTCTGCGCCAGGCTCGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 445 |
| LEAP-Seq percent confirming: | 99.8354 |
| LEAP-Seq n confirming: | 1213 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCGTGTGTGTGTGTGTGTGT |
| Suggested primer 2: | AGTCCTGCGTGCTCCTTTTA |