| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.211579 |
| Chromosome: | chromosome 9 |
| Location: | 1017470 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g400851 | (1 of 781) IPR000104 - Antifreeze protein, type I | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCGTCGGCCTGCCGGGGACTGTAACAAGC |
| Internal bar code: | GACTGGCCGGGGAATCGTCCTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 498 |
| LEAP-Seq percent confirming: | 99.7714 |
| LEAP-Seq n confirming: | 2182 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCCTTGATCTAGCGCGATTT |
| Suggested primer 2: | GTAGCTGCGCTCCATACACA |