| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.211600 |
| Chromosome: | chromosome 17 |
| Location: | 3464893 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g724873 | 3'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTGGGAGCACAAGAGCAAAAGCACCCCCA |
| Internal bar code: | GCCCGGTTTCGCACTTCCGCAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 659 |
| LEAP-Seq percent confirming: | 98.6721 |
| LEAP-Seq n confirming: | 7802 |
| LEAP-Seq n nonconfirming: | 105 |
| LEAP-Seq n unique pos: | 33 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCAGAGATTACGGCAGCAT |
| Suggested primer 2: | CGTGCACCTTACACGATTTG |