Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.211613 |
Chromosome: | chromosome_10 |
Location: | 3991785 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre10.g448850 | FAP135,ODA5AK,CAL5 | Calpain cystein protease and Flagellar Associated Protein | sense | CDS |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | CCTGAACGGCGGCTGCCACTACGTCATCAT |
Internal bar code: | CAGAAGAGAAGTGTGGGCACGG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 1019 |
LEAP-Seq percent confirming: | 99.716 |
LEAP-Seq n confirming: | 12989 |
LEAP-Seq n nonconfirming: | 37 |
LEAP-Seq n unique pos: | 59 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | ACACACACACGCACACACAC |
Suggested primer 2: | ACGTTGCCTCTTGGAGAGAA |