Insertion junction: LMJ.RY0402.211634_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.211634
Chromosome:chromosome 9
Location:3490535
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre09.g389652 (1 of 1) PTHR31437//PTHR31437:SF1 - FAMILY NOT NAMED // PROTEIN SREK1IP1 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCAAACAGTTGCGCTTTCATCAGCTGTTAC
Internal bar code:TTCTAACCGCAGTGATGCTTGT

Confirmation - LEAP-Seq

LEAP-Seq distance:803
LEAP-Seq percent confirming:99.604
LEAP-Seq n confirming:2264
LEAP-Seq n nonconfirming:9
LEAP-Seq n unique pos:9

Suggested primers for confirmation by PCR

Suggested primer 1:GAAGAGGACGATAGGGAGGG
Suggested primer 2:ATCTTGCACTGCGTTGACTG