Insertion junction: LMJ.RY0402.211635_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.211635
Chromosome:chromosome 11
Location:564446
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre11.g467608 (1 of 4) IPR003882//IPR008752 - Pistil-specific extensin-like protein // Peptidase M11, gametolysin intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACTCCCGTTGCCAGCGGATCAGGGACAAAG
Internal bar code:CCTGGGAACACCATGCTTGCGC

Confirmation - LEAP-Seq

LEAP-Seq distance:355
LEAP-Seq percent confirming:52.1463
LEAP-Seq n confirming:2466
LEAP-Seq n nonconfirming:2263
LEAP-Seq n unique pos:8

Suggested primers for confirmation by PCR

Suggested primer 1:CGATGATGTTGTTGTCTGGG
Suggested primer 2:ACTTCCTCTTCCCCACAGGT