| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.211642 |
| Chromosome: | chromosome 14 |
| Location: | 249426 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g609350 | (1 of 24) PTHR10641 - MYB-LIKE DNA-BINDING PROTEIN MYB | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGTTCAAGGCCATGCTCTTCGAGGCTCGG |
| Internal bar code: | CTCAGCTCATTGACCGCGTCAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 436 |
| LEAP-Seq percent confirming: | 99.359 |
| LEAP-Seq n confirming: | 155 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGGAGAGCAAGAAGAACGC |
| Suggested primer 2: | GCTGAGTGTGTGTGCATGTG |