| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.211644 |
| Chromosome: | chromosome 1 |
| Location: | 3354580 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g021200 | CGL47 | conserved protein with F-box domain; (1 of 10) IPR000104//IPR001810 - Antifreeze protein, type I // F-box domain | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGAAGGGAGCCAAAGGGCAGCAGCGCGGC |
| Internal bar code: | GGCCTTTGGCACAGCGCAGGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 313 |
| LEAP-Seq percent confirming: | 99.2466 |
| LEAP-Seq n confirming: | 7640 |
| LEAP-Seq n nonconfirming: | 58 |
| LEAP-Seq n unique pos: | 29 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAGGCTTATAGGAAGGCGCA |
| Suggested primer 2: | CACGTACGCCTGCATGTATC |