| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.211668 |
| Chromosome: | chromosome 1 |
| Location: | 3032642 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g018650 | (1 of 1) PF01342//PF13771 - SAND domain (SAND) // PHD-like zinc-binding domain (zf-HC5HC2H) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGCGCACGGTGTCCAGATGAAATAAAAGA |
| Internal bar code: | ACAGCGGACCTACTCACAGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1145 |
| LEAP-Seq percent confirming: | 98.5277 |
| LEAP-Seq n confirming: | 4350 |
| LEAP-Seq n nonconfirming: | 65 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTTACGGGTATGGTGAGCG |
| Suggested primer 2: | CGACTACACCGGAGCTTCTC |