| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.211668 |
| Chromosome: | chromosome 1 |
| Location: | 3032864 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| intergenic |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCAGCATGCAGCAACTCACCATGCCCACA |
| Internal bar code: | ACGGTATCAGGTGGTCGATGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 961 |
| LEAP-Seq percent confirming: | 89.3775 |
| LEAP-Seq n confirming: | 9533 |
| LEAP-Seq n nonconfirming: | 1133 |
| LEAP-Seq n unique pos: | 63 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GATGCAGAGGATCGGTGTTT |
| Suggested primer 2: | CGACTACACCGGAGCTTCTC |