Insertion junction: LMJ.RY0402.211681_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.211681
Chromosome:chromosome 1
Location:2445426
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre01.g013650 (1 of 3) PF15612 - WSTF, HB1, Itc1p, MBD9 motif 1 (WHIM1) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTCATGTCGACTCCGCCACCTGCGGCCGCG
Internal bar code:ACGGGAAGCCAACGCAAAATGA

Confirmation - LEAP-Seq

LEAP-Seq distance:390
LEAP-Seq percent confirming:92.3345
LEAP-Seq n confirming:530
LEAP-Seq n nonconfirming:44
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:CCCTACCCAAACAGACTCCA
Suggested primer 2:CGGCAGTACCAGACTTGTCA