| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.211722 |
| Chromosome: | chromosome_10 |
| Location: | 5166648 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre10.g456600 | antisense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TTGTTGCTCCCCTGCAGAGCAGGCCACACG |
| Internal bar code: | CAACGGGGCGCCTTAAAAGTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1090 |
| LEAP-Seq percent confirming: | 99.2407 |
| LEAP-Seq n confirming: | 8888 |
| LEAP-Seq n nonconfirming: | 68 |
| LEAP-Seq n unique pos: | 66 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCAGAAGGTGGTGAACAGGT |
| Suggested primer 2: | GCCACGCTCATCTAGGACTC |