| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.211732 |
| Chromosome: | chromosome 10 |
| Location: | 3276433 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g443300 | CNK5 | NimA-related protein kinase; (1 of 1) PTHR24361//PTHR24361:SF368 - MITOGEN-ACTIVATED KINASE KINASE KINASE // SUBFAMILY NOT NAMED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCGCCCAGCTTGATGATGCCCCCCTTGGC |
| Internal bar code: | CTACGACTGTAGGTTTTTCATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 415 |
| LEAP-Seq percent confirming: | 92.3977 |
| LEAP-Seq n confirming: | 158 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTTCTTCTTTCGGGCTCTT |
| Suggested primer 2: | CCATGTTACTTGCATGGTGC |