| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.211732 |
| Chromosome: | chromosome 12 |
| Location: | 4668261 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g523150 | PFD5 | Prefoldin alpha subunit; (1 of 1) K04797 - prefoldin alpha subunit (pfdA, PFDN5) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCAGCCGCATTGGCAGCGTGGGAACCACTG |
| Internal bar code: | AGATAAAAGTGGATTTAGTGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1102 |
| LEAP-Seq percent confirming: | 99.525 |
| LEAP-Seq n confirming: | 7333 |
| LEAP-Seq n nonconfirming: | 35 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGACGCTGCAGGAGAACATT |
| Suggested primer 2: | TGGCGTCAAACATCTACGAG |