| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.211732 |
| Chromosome: | chromosome 12 |
| Location: | 4668330 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g523150 | PFD5 | Prefoldin alpha subunit; (1 of 1) K04797 - prefoldin alpha subunit (pfdA, PFDN5) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCAGACCCCAGCAGATGGCAGACACCTTG |
| Internal bar code: | TTTCTGGCACGCAACTCTGGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 482 |
| LEAP-Seq percent confirming: | 32.9479 |
| LEAP-Seq n confirming: | 1738 |
| LEAP-Seq n nonconfirming: | 3537 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTTCTTGCAGACCCTGAAC |
| Suggested primer 2: | TGGCGTCAAACATCTACGAG |