Insertion junction: LMJ.RY0402.211743_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.211743
Chromosome:chromosome 4
Location:2934454
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre04.g224650 FAP296 (1 of 1) PTHR23316//PTHR23316:SF0 - IMPORTIN ALPHA // IMPORTIN SUBUNIT ALPHA; Flagella-Associated Protein 296, similar to Importin Alpha 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGGCGGGGCGTCTCCGCCCATCGCTCGCGG
Internal bar code:TCGGTGCAGACTCCGTCTCGCT

Confirmation - LEAP-Seq

LEAP-Seq distance:1077
LEAP-Seq percent confirming:98.3516
LEAP-Seq n confirming:1432
LEAP-Seq n nonconfirming:24
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:AGTGAGGTTAGAGCCAGGCA
Suggested primer 2:TGAGCTGACTGGTATCGTGC