| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.211750 |
| Chromosome: | chromosome 12 |
| Location: | 4481403 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g521700 | SSS6,SSS1A | Soluble starch synthase IA; (1 of 5) K00703 - starch synthase (E2.4.1.21, glgA) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AACCGCATCCTGAGCGTCGCCTTACACGAC |
| Internal bar code: | CTCGCTCTAGTTGCCGCAGTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1073 |
| LEAP-Seq percent confirming: | 99.4141 |
| LEAP-Seq n confirming: | 3563 |
| LEAP-Seq n nonconfirming: | 21 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGGACAGTGAATTGGCGAT |
| Suggested primer 2: | CCTCCCTCCTAGCCATCCTA |