| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.211772 |
| Chromosome: | chromosome 8 |
| Location: | 4937255 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g385350 | (1 of 5) PTHR32467//PTHR32467:SF15 - FAMILY NOT NAMED // AP2-LIKE ETHYLENE-RESPONSIVE TRANSCRIPTION FACTOR SMZ-RELATED | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTAATGGCGCCTTGCCTGTCTCTCCTTGCA |
| Internal bar code: | TTCACACGTGTACGTAAGGGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 510 |
| LEAP-Seq percent confirming: | 97.5 |
| LEAP-Seq n confirming: | 6357 |
| LEAP-Seq n nonconfirming: | 163 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTACACCTACTGCACCAGCG |
| Suggested primer 2: | GTTCGCTTCAGTACCTTCGC |