Insertion junction: LMJ.RY0402.211775_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.211775
Chromosome:chromosome 12
Location:8251088
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre12.g550300 (1 of 1) PTHR24361//PTHR24361:SF276 - MITOGEN-ACTIVATED KINASE KINASE KINASE // PROTEIN KINASE intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGGCAGAACTGTAATGTTTACTCGTGTGAG
Internal bar code:ATTTTTTTCGACCAGGTGCTAT

Confirmation - LEAP-Seq

LEAP-Seq distance:203
LEAP-Seq percent confirming:99.8138
LEAP-Seq n confirming:536
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:GAGTGGTGGTGGGAGTGTCT
Suggested primer 2:GTGCTGCTGTGACAGTTGGT