Insertion junction: LMJ.RY0402.211776_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.211776
Chromosome:chromosome 2
Location:2719562
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre02.g093600 TEH3 (1 of 6) PF03061 - Thioesterase superfamily (4HBT); Thioesterase-like protein CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCCCCTCAGCAATGTCGTGTGGCTGCACCA
Internal bar code:AGGCCGCGTTATGGTAGCGGAG

Confirmation - LEAP-Seq

LEAP-Seq distance:322
LEAP-Seq percent confirming:92.3891
LEAP-Seq n confirming:3666
LEAP-Seq n nonconfirming:302
LEAP-Seq n unique pos:8

Suggested primers for confirmation by PCR

Suggested primer 1:CAGTAGCCCTTGTCGGTAGC
Suggested primer 2:CAGTGAGTCCTAGCCAAGCC