Insertion junction: LMJ.RY0402.211776_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.211776
Chromosome:chromosome 11
Location:1152489
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre11.g467685 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTTGGGCGCCGACCCCTCGGCAGTCACGGT
Internal bar code:CTCGATATATTCTTGCACCATA

Confirmation - LEAP-Seq

LEAP-Seq distance:682
LEAP-Seq percent confirming:99.7172
LEAP-Seq n confirming:1058
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:8

Suggested primers for confirmation by PCR

Suggested primer 1:ACAACGCTTATCCAACCTGG
Suggested primer 2:GCAATCTAGTTTGGTCCCCA