| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.211777 |
| Chromosome: | chromosome 4 |
| Location: | 3575429 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g228500 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCAATCCCTCAAAGCCCTAACACCTGGCGA |
| Internal bar code: | TAATGCAGGTCTAACAGCCGAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 576 |
| LEAP-Seq percent confirming: | 70.9845 |
| LEAP-Seq n confirming: | 685 |
| LEAP-Seq n nonconfirming: | 280 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCACTAGCATCCAGCATGAA |
| Suggested primer 2: | AATGGAGATCTGTGCCCAAG |