| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.211790 |
| Chromosome: | chromosome 11 |
| Location: | 2683677 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g476700 | MPA9 | Metallophosphoesterase/metallo-dependent phosphatase; (1 of 3) 3.1.3.26 - 4-phytase / Phytate 6-phosphatase | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AATTTGGGGTTCGTAGGTGCAGCCCTCCCT |
| Internal bar code: | AACGGGAGGAGGGCTTTTGTCCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2225 |
| LEAP-Seq percent confirming: | 97.5035 |
| LEAP-Seq n confirming: | 2070 |
| LEAP-Seq n nonconfirming: | 53 |
| LEAP-Seq n unique pos: | 30 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGAATGGACATAGGGGTCC |
| Suggested primer 2: | GCAAGAAGAAGGTCCAGACG |