| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.211797 |
| Chromosome: | chromosome 13 |
| Location: | 3740815 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g589426 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTGGCGAGATTCGGGGACCGCATGCAGCC |
| Internal bar code: | CAACGTTTGCAAGCCTGCCCTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 758 |
| LEAP-Seq percent confirming: | 97.946 |
| LEAP-Seq n confirming: | 8345 |
| LEAP-Seq n nonconfirming: | 175 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGAGTGAAAGCGAGTTGTG |
| Suggested primer 2: | CGGGATACATTCCAATACGG |