| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.211802 |
| Chromosome: | chromosome 12 |
| Location: | 215938 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g484900 | FAP83 | (1 of 2) PF13879 - KIAA1430 homologue (KIAA1430); Flagellar Associated Protein 83 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGGGCATAGCAGCAGCCAAACCCTGGTGT |
| Internal bar code: | GTGGGTAAGCTCAGCAGGGCGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 943 |
| LEAP-Seq percent confirming: | 99.7688 |
| LEAP-Seq n confirming: | 13811 |
| LEAP-Seq n nonconfirming: | 32 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGGCTTACAGACATGAAGCA |
| Suggested primer 2: | GCTCCTCGTCCTGCTCATAC |