| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.211809 |
| Chromosome: | chromosome 3 |
| Location: | 2430843 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g159200 | UBC8 | (1 of 2) K10589 - ubiquitin-protein ligase E3 C (UBE3C); Putative ubiquitin-protein ligase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGATGTCACGGAGGAGTGGAGGACCACTCA |
| Internal bar code: | CGGGATTGGTTCTCTTTTTTCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 613 |
| LEAP-Seq percent confirming: | 98.8481 |
| LEAP-Seq n confirming: | 1373 |
| LEAP-Seq n nonconfirming: | 16 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTCTGTGACCAAGTTGGGG |
| Suggested primer 2: | AAGGAAAAGCACAGCCTGAA |