| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.211813 |
| Chromosome: | chromosome 3 |
| Location: | 4766300 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g178150 | RSP20,CAM1 | Calmodulin, radial spoke protein | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCTGCAGGGGCCAAGAAGCTATTTGCGTC |
| Internal bar code: | CCGCTGGGCGAATTGCGGATGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1030 |
| LEAP-Seq percent confirming: | 99.6433 |
| LEAP-Seq n confirming: | 8102 |
| LEAP-Seq n nonconfirming: | 29 |
| LEAP-Seq n unique pos: | 48 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGGGACAATGGAGCAGGTA |
| Suggested primer 2: | ATTGACCGTGTCCTACCTGC |