| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.211814 |
| Chromosome: | chromosome 12 |
| Location: | 5504856 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g531050 | RAA3,OPR67 | psaA mRNA trans-splicing factor; (1 of 45) IPR013584 - RAP domain | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTGACCACCGCCTGCTCAGCGCCTTGCAC |
| Internal bar code: | GAATACCTGGATCAGGACAGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 771 |
| LEAP-Seq percent confirming: | 93.1213 |
| LEAP-Seq n confirming: | 3425 |
| LEAP-Seq n nonconfirming: | 253 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAATATCATCAGCGCCGAAC |
| Suggested primer 2: | CCTCCTCATACCACCTCCAA |