| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.211838 |
| Chromosome: | chromosome 5 |
| Location: | 923058 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g247250 | (1 of 781) IPR000104 - Antifreeze protein, type I | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGAGGCAATTGTGCTTGACACAAAACACGC |
| Internal bar code: | AGTGTCCTTACTAAGGTTTTAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 777 |
| LEAP-Seq percent confirming: | 98.9834 |
| LEAP-Seq n confirming: | 2142 |
| LEAP-Seq n nonconfirming: | 22 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGTGTGTACGGGCGTGTAG |
| Suggested primer 2: | CGTCGTCTGTGTAACATGGG |