| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.211856 |
| Chromosome: | chromosome 10 |
| Location: | 1267320 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g426900 | (1 of 3) PF02458 - Transferase family (Transferase) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTCCGAAAACCTCTCCGTGCTTACTTGCG |
| Internal bar code: | GATGACTTTGAGTCTTCTTTCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 540 |
| LEAP-Seq percent confirming: | 80.5009 |
| LEAP-Seq n confirming: | 450 |
| LEAP-Seq n nonconfirming: | 109 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTGCGTGTTGCTGTAGTGT |
| Suggested primer 2: | GGCAGTCTTAGAAGCGGTTG |