| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.211867 |
| Chromosome: | chromosome 4 |
| Location: | 3971512 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g231614 | (1 of 1) IPR001611//IPR003591//IPR023353 - Leucine-rich repeat // Leucine-rich repeat, typical subtype // LemA-like domain | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCTTCTGCATCGATGGCCGTGTTGTTTCA |
| Internal bar code: | TGCCAAGCGCGCGACGTAGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 169 |
| LEAP-Seq percent confirming: | 74.1936 |
| LEAP-Seq n confirming: | 23 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAGGCAACTGTCAGACACGG |
| Suggested primer 2: | AACCTTCATGACTGGATCGC |